华北农学报2009，Vol.24Issue(5)：69-72,4.
花生磷酸烯醇式丙酮酸羧化酶基因的克隆及反义表达载体的构建
Cloning of Peanut Gene Fragment Encoding PEPCase and Construction of Its Plant Expression Vector
摘要
Abstract
Phosphoenolpyruvate carboxylase (PEPCase) plays an important role in the control of the ratio of the pro-tein/lipid content in plant seed. A 886 bp DNA fragment which was amplified by RT-PCR using PEPCase gene special primer from peanut seed. The DNA sequence analysis indicated that the sequence of this fragment was shared 99.77 % , 84.37%, 81. 54% ,81.25% ,78. 71% homology with the corresponding sequence of the reported PEPCase gene in peanut,cotton,soybean,thale cress,pea.The 886 bp fragment digested by BamH I and Sac I and ligated into the GUS site of the pBI121 in the antisense orientation and constructed the antisence expression vector pBGPEP.关键词
花生/PEP羧化酶/RT-PCR/反义表达载体Key words
Peanut/ Phosphoenolpyruvate carboxylase/ Reverse transcription-polymerse chain reaction/ Antisence ex-pression vector分类
生物科学引用本文复制引用
潘昱名,刘风珍,万勇善..花生磷酸烯醇式丙酮酸羧化酶基因的克隆及反义表达载体的构建[J].华北农学报,2009,24(5):69-72,4.基金项目
国家"863"计划项目(2006AA10A115) （2006AA10A115）
山东省中青年科学家科研奖励基金项目(2006BSB01049) （2006BSB01049）
山东博士后创新项目(200703034) （200703034）
