华北农学报2009，Vol.24Issue(5)：73-76,4.
黑曲霉木聚糖酶结构基因和5'调控区基因克隆及其分析
Cloning and Sequence Analysis of β-xylanase Structural Gene and the 5'Flanking Regions Gene from Aspergillus niger
摘要
Abstract
In this paper,the cloning of a β-xylanase structural gene and its 5'flanking regions gene from Aspergillus niger was studied. On the base of nucleic acid DNA sequence of β-xylanase gene, a pair of degenerate primers was de-signed . Using the genomic DNA of Aspergillus niger as template, a 1.6 kb fragment was amplified by PCR and cloned into the vector pBS-T. Sequence analysis showed that the structural gene of β-xylanase is 744 bp length including a 66 bp in-tron and a 678 bp encoding region, which encoded a polypeptide of 225 amino acids and included the signal sequence of 18 amino acids.The homology analysis of the xynB cDNA sequence shares 98% in base sequence with the β-xylanase gene in GeneBank and the amino acid sequence shares 99 % . Upstream of the tranlation start site, there were classic core element of Eukaryotic Gene Promoter,such as transcription start site,TATA box and CAAT box elements.The work was beneficial in construction of homologus expression for xylanase efficient expression vector.关键词
黑曲霉/木聚糖酶/基因克隆/序列分析Key words
Aspergillus niger/ Xylanase/ Gene cloning/ Sequence analysis分类
化学化工引用本文复制引用
白爱枝,闫祖威,唐国敏,梁运章..黑曲霉木聚糖酶结构基因和5'调控区基因克隆及其分析[J].华北农学报,2009,24(5):73-76,4.基金项目
国家自然科学基金资助项目(10465002) （10465002）
