华北农学报2009，Vol.24Issue(5)：192-196,5.
大豆炭腐病病原菌鉴定
Identification of Pathogen Causing Soybean Charcoal Rot
摘要
Abstract
Charcoal rot,causing by Macrophomina phaseolina (Tassi)Goid is an important soybean disease in the world. In late August 2005 and 2007, symptoms of charcoal rot-like were observed on soybean plants in one field in Tian-jin and Beijing, respectively. Both fields had more than 80 % disease incidence, with extensive premature death prior to pod-filling stage. Fungal isolations were made from the diseased stem tissue in order to identify the causal agent. Five re-sulting isolates were cultured on potato dextrose agar at 30℃ ,and were identified as M.phaseolina based on the gray col-or of the colony and the presence of microsclerotia 49.8 to 111.4 μm in size.Pathogenicity was performed by inoculation of 2-week-old seedlings of cv. Hefeng 25 using hypocotyl inoculation and cut stem techniques. All inoculated plants showed dark brown to black lesions. For each isolate tested, M. phaseolina was resolated from inoculated plants. To con-firm the morphological identification, the rDNA-ITS region of the five isolates was amplified with universal primer ITS4 and ITS5.The obtained ITS sequences (one sequence Gen Bank accession No. FJ175382) were aligned with GenBank and showed 97 % - 99 % similarity with isolates of M. phaseolina . PCR specific primers MpKFl and MPKR1 for M. phaseoli-na were used to detect genome DNA of the five isolates, the primers produced single amplified product of 350 bp with the five isolates, and the results confirmed them as M. phaseolina . Up to now, only two notes about its occurrence were pub-lished in Liaoning Province and Shandong Province, and the causing pathogen did not have been identified. This is the first report of occurrence of charcoal rot of soybean in Northern China.关键词
大豆/炭腐病/菜豆壳球孢菌/鉴定Key words
Glycine max/ Charcoal rot/ Macrophomina phaseolina/ Identification分类
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张吉清,崔友林,段灿星,武小菲,王晓鸣,朱振东..大豆炭腐病病原菌鉴定[J].华北农学报,2009,24(5):192-196,5.基金项目
中国农业科学院作物科学研究所中央级公益性科研院所基本科研业务费专项(082060302-06) （082060302-06）
