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喜树内生真菌的分离及产10-羟基喜树碱菌的鉴定

闵长莉 袁萍

华东师范大学学报(自然科学版)Issue(5):94-99,6.
华东师范大学学报(自然科学版)Issue(5):94-99,6.

喜树内生真菌的分离及产10-羟基喜树碱菌的鉴定

Isolation and identification of a 10-hydroxycamptothecin-producing endophytic fungus from Camptotheca acuminate Decne

闵长莉 1袁萍2

作者信息

  • 1. 皖西学院化学与生命科学系,安徽六安237012
  • 2. 上海师范大学生命与环境科学学院,上海200234
  • 折叠

摘要

Abstract

Six endophytic fungi isolated from Camptotheca acuminate Decne were cultured in taper flask under shaking. The organic extracts of mycelium were analyzed with thin layer chroma-tography (TLC) and high performance liquid chromatography (HPLC). The results showed one of the strains produced the 10-hydroxycamptothecin (HCPT) with output of 410 f*g/L and was named XK002. Morphological observation indicates that XK002 belongs to Mycelia sterlia.

关键词

喜树内生真菌/分离/鉴定/10-羟基喜树碱

Key words

endophytic fungi of Camptotheca acuminate Decne/ isolation/ identification/ 10-hydroxycamptothecin

分类

生物科学

引用本文复制引用

闵长莉,袁萍..喜树内生真菌的分离及产10-羟基喜树碱菌的鉴定[J].华东师范大学学报(自然科学版),2009,(5):94-99,6.

华东师范大学学报(自然科学版)

OA北大核心CSCDCSTPCD

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