首页|期刊导航|哈尔滨工业大学学报（英文版）|Cloning and expression in Saccharomyces cerevisiae of chit2 gene from Beauveria bassiana
哈尔滨工业大学学报（英文版）2009，Vol.16Issue(4)：453-456,4.
Cloning and expression in Saccharomyces cerevisiae of chit2 gene from Beauveria bassiana
Cloning and expression in Saccharomyces cerevisiae of chit2 gene from Beauveria bassiana
摘要
Abstract
To study recycled trashes from shrimps and crabs in the sea through chitinase secreted by microor-ganisms, the chitinase gene chit2 was cloned and sequenced from Beauveria bassiana by the polymerase chain reaction (PCR), and was ligated into the yeast expression vector pYES2. The expression vector plasmid was transformed into Saccharomyces cerevisiae H158. Gene expression took place upon induction with 2% galac-tose. The measurement of enzyme activity shows that the expression production can be expressed in active forms and secreted to the medium. The enzyme activity approaches the peak of 0. 63 U/mL when the culture time is 36 h.关键词
Beauveria bassiana / ehitinase/ Saccharomyces cerevisiae / expressionKey words
Beauveria bassiana / ehitinase/ Saccharomyces cerevisiae / expression分类
生物科学引用本文复制引用
SONG Jin-zhu,YANG Xiao-xue,WANG Yun,YANG Qian..Cloning and expression in Saccharomyces cerevisiae of chit2 gene from Beauveria bassiana[J].哈尔滨工业大学学报（英文版）,2009,16(4):453-456,4.基金项目
Sponsored by the Natural Science Foundation of Heilongjiang Province (Grant No. C200609) and the National Science and Technology Supported Pro-grame (Grant No. 2006BAD07A01). （Grant No. C200609）
