首页|期刊导航|哈尔滨工业大学学报(英文版)|Genomic and proteomic analysis of soybean heritable variations induced by space flight
哈尔滨工业大学学报(英文版)2009,Vol.16Issue(4):587-592,6.
Genomic and proteomic analysis of soybean heritable variations induced by space flight
Genomic and proteomic analysis of soybean heritable variations induced by space flight
摘要
Abstract
To analyze the biological effects of space environment, the diversity of genomic DNA between the space flight soybean 194(4126) with phenotype of good yield and good fruit quality induced by space flight and the soybean with ground control was studied by amplified fragment length polymorphism (AFLP) method, and the polymorphism of space flight soybean 194(4126) was 3.56%. The differences of protein expression of seeds and leaves between the two kinds of soybeans were analysed by two-dimensional electrophoresis, PDQuest software and MALDI-TOF mass spectrometry. Results show that the loss and decrease of protein expression in 194(4126) soybean are subjected to the space fight of seeds, and three special proteins including Dehydrin, MAT1 and ceQORH are identified. It is concluded that the space environment changes the phenotype and geno-type of soybeans due to the space flight of seeds.关键词
space environment/ genomic / proteomic / soybeanKey words
space environment/ genomic / proteomic / soybean分类
生物科学引用本文复制引用
HE Jie,GAO Yong,SUN Ye-qing..Genomic and proteomic analysis of soybean heritable variations induced by space flight[J].哈尔滨工业大学学报(英文版),2009,16(4):587-592,6.基金项目
Sponsored by the National High Technology Research and Development Program of China (Grant No. 2004AA231071) and the Fund of Harbin Institute of Technology (Grant No. HITQNJS. 2006. 048 and HIT. 2000. 10). (Grant No. 2004AA231071)