河南农业大学学报2009，Vol.43Issue(5)：540-542,547,4.
小麦禾谷胞囊线虫不同抗性品种中几丁质酶基因Cht4的表达分析
Expression analysis of wheat chitinase gene Cht4 in the resistance and susceptible varieties to cereal cyst nematode
摘要
Abstract
Expression analysis about wheat chitinase gene,in the different growing stages and different positions of different resistance cultivars after CCN inoculation, was carried by semi-Q RT-PCR, using wheat Actin gene as a control. The result showed that the quantity of chitinase gene expression obvious increased after 12 h inoculation, and there were no obvious differences between resistant and susceptible cultivars. After 36 h inoculation, the expression quantity in both cultivars increased obviously, and the quantity in resistant cultivars was more than susceptible cultivars, and in leaves was more than in roots. After 60 h inoculation, the gene expression quantity in resistant and susceptible cultivars was keeping a high level, and the former was more than the latter. The expression in susceptible cultivars after 84 h inoculation didn' t have obvious difference from the ones without inoculation, but the expression quantity in resistant cultivars was still higher than those without inoculation.RT-PCR;chitinase gene关键词
小麦/禾谷胞囊线虫/半定量RT-PCR/几丁质酶基因Key words
wheat/cereal cyst nematode/semi-Q分类
农业科技引用本文复制引用
殷宪平,李洪连,汪敏,袁虹霞..小麦禾谷胞囊线虫不同抗性品种中几丁质酶基因Cht4的表达分析[J].河南农业大学学报,2009,43(5):540-542,547,4.基金项目
国家粮食丰产工程河南课题(2006BAD02A07-2) （2006BAD02A07-2）
河南省重大公益性科研招标项目(081100911300) （081100911300）
