河南农业大学学报2009，Vol.43Issue(5)：552-556,5.
苦瓜核糖体失活蛋白MAP30的原核表达与抗体制备
Prokaryotic expression and antibody preparation of ribosome-inactivating protein MAP30
摘要
Abstract
Ribosome-inactivating proteins MAP30 in bitter melon(Momordica charantia L. ) is a protein with anti-virus, anti-tumor, anti-bacterial and other biological activities. The recombinant plasmid pET-28(a)-MAP30 was transformed into E. coli BL21 and induced to express by IPTG. After induced by 0.2 mmol ·L~(-1) IPTG at 30 ℃ for 6 h, the fusion protein His-MAP30 was produced in high-level and soluble form. The fusion protein was purified by Ni~(2+)-NTA column. The target protein was eluted efficiently when the imidazole concentration of elution buffer was 100 mmol ·L~(-1). The specific poly-clonal antibodies have been raised in rabbits immuned with the purified recombinant protein.关键词
苦瓜/苦瓜核糖体失活蛋白/原核表达/抗体制备Key words
bitter melon/ribosome inactivating protein MAP30/prokaryotic expression/antibody preparation分类
生物科学引用本文复制引用
许君,乔帅,常岚,朱海华,郑文明..苦瓜核糖体失活蛋白MAP30的原核表达与抗体制备[J].河南农业大学学报,2009,43(5):552-556,5.基金项目
河南农业大学博十研究基金项目(30200274) （30200274）
