化工学报2009，Vol.60Issue(11)：2798-2804,7.
乙醇/碳酸钾双水相萃取盾叶薯蓣发酵液中的2,3-丁二醇
Aqueous two-phase extraction of 2,3-butanediol by ethanol/potassium carbonate system from Dioscorea zingiberensis fermentative broths
摘要
Abstract
Aqueous two-phase extraction of 2,3-butanediol from fermented broths was investigated by using ethanol/potassium carbonate system. The bioconversion of saccharified hydrolyzate of Dioscorea zingiberensis into 2,3-butanediol was carried out by Klebsiella pneumoniae. The partitioning experiment indicated that the optimal conditions for aqueous two-phase extraction of 2,3-butanediol from the fermentation broth clarified by hollow fiber membrane were 22% (mass) of ethanol and 26% (mass) of potassium carbonate. Under this optimal condition, aqueous two-phase extraction of 2,3-butanediol was carried out directly from the fermentation broth without filtration. The recoveries of 2,3-butanediol and acetoin in the top phase as well as reducing sugar in the bottom phase were 97%, 97% and 87%, respectively. The removal rates of cells and proteins were 99% and 94% , respectively. Pyruvic acid, citric acid, malic acid, fumaric acid and butane diacid were partitioned into the bottom phase. Aqueous two-phase system of ethanol/potassium carbonate exhibited a great potential in the industrial separation of 2,3-butanediol.关键词
2/3-丁二醇/双水相萃取/有机酸/发酵液Key words
2/3-butanediol/ aqueous two-phase extraction/ organic acids/ fermentative broths分类
生物科学引用本文复制引用
刘国兴,江波,王元好,戴建英,修志龙..乙醇/碳酸钾双水相萃取盾叶薯蓣发酵液中的2,3-丁二醇[J].化工学报,2009,60(11):2798-2804,7.基金项目
教育部优秀人才基金项目(NCET-05-0279) （NCET-05-0279）
国家高技术研究发展计划项目(2009AA05Z443). （2009AA05Z443）
