化工学报2009，Vol.60Issue(12)：3063-3070,8.
重组大肠杆菌HT02高密度高表达HT-1融合蛋白发酵过程优化
Optimization of high cell culture of recombinant E. coli HT02 for high expression of HT-1 fusion protein
胡爽 1蔡海波 1蒋加庆 1谭文松1
作者信息
- 1. 华东理工大学生物反应器工程国家重点实验室,上海,200237
- 折叠
摘要
Abstract
The scale-up of fermentation process plays an important role in the industrialization of lab results. As the increase of fermentation scale, the microenvironment of microorganism would be different, which would result in the change of microorganism metabolism and affect the results of scale-up. In order to accomplish the industrialization of HT-1 production, high-expression of HT-1 fusion protein was carried out with fed-batch culture method of E. Coli in a 200 L fermentor. The problem of the decrease in HT-1 fusion protein expression level caused by secondary seed culture process was solved by the methods of advancing initial induction and increasing inoculation volume. As a result, the cell density, the expression level, the cell yield as well as the volume yield of HT-1 fusion protein were achieved to 55. 1 g · L~(-1) , 27.4%, 0.056 g · (g cell)~(-1) and 3.103 g · L~(-1), respectively. Compared with the results before optimization, these indexes were increased by 9.8%, 23.4%, 14.3% and 25.2%, respectively. These results provide an important reference for the optimization and scale-up of HT-1 production.关键词
重组大肠杆菌/高密度发酵/大规模培养Key words
recombinant E. Coli/ high cell-density fermentation/ large-scale culture分类
生物科学引用本文复制引用
胡爽,蔡海波,蒋加庆,谭文松..重组大肠杆菌HT02高密度高表达HT-1融合蛋白发酵过程优化[J].化工学报,2009,60(12):3063-3070,8.
