黑龙江农业科学Issue(6)：4-8,5.
巴西橡胶树核糖体蛋白S6基因的克隆及生物信息学分析
Cloning and Bioinformatic Analysis of the Gene Encoding the Ribosomal Protein S6 from Hevea brasiliensis
摘要
Abstract
The ribosomal protein S6 is the key member of ribosomal protein family,and it plays an important role in the ribosomal proteins.The primers designed based on the known sequences acquired from Key Laboratory of Rubber Bidoye of Minisby of Agriculture,the gene was cloned with 3'-RACE technique from Hevea brasiliensis latex.The open reading frame encoded 249 amino acids,containing a conserved ribosomal S6 domain,so the gene was named as HbRPS6.No signal peptide and transmembrane structure were predicted within HbRPS6.The result of secondary structure predicted showed that the protein was classified as mixed type.The result of phylogenetic tree indicated that HbRPS6 displayed a high degree of sequence similarity to ZmRPS5,but not to animals and yeast.This paper will be benefit to further analyze the functions of HbRPS6.关键词
巴西橡胶树/核糖体蛋白S6/基因克隆/序列分析Key words
Hevea brasiliensis/ribosomal protein S6/gene cloning/sequence analysis分类
农业科技引用本文复制引用
李德军,邓治,陈春柳,陈守才..巴西橡胶树核糖体蛋白S6基因的克隆及生物信息学分析[J].黑龙江农业科学,2009,(6):4-8,5.基金项目
国家天然橡胶现代农业产业技术体系建设项目(nycytx-34-GW1-2) （nycytx-34-GW1-2）
中国热带农业科学院橡胶研究所基本科研业务费专项资金资助项目(XJSYWFZX2009-08) （XJSYWFZX2009-08）
