湖南大学学报（自然科学版）2009，Vol.36Issue(11)：67-72,6.
不同激活剂条件下油藏内源微生物激活过程中DGGE分析
Denature Gradient Gel Electrophoresis of Strata Bacteria Activation in Oilfield under Different Activating Nutrient Conditions
摘要
Abstract
On the basis of analyzing the reservoir status of ZHAN 3 block in Shengli Oilfield, different nutrients were used to activate the indigenous microorganisms by utilizing the denature gradient gel elec-trophoresis (DGGE) under the condition of stratum. The results showed that the microbial communities changed noticeably when glucose, cane sugar, corn steep liquor, starch solution were used as the carbon source,and the produced water and injected water were used as the objects. In addition, based on the effect and the practicability, the concentration of glucose was altered in further experiments, which indicated that the density of the indigenous microorganisms could reach 107 cell·mL~(-1) when the concentration of glucose was from 5 g·L~(-1) to 7.5 g·L~(-1), and the results also indicated that the produced water was better than the injected water for microbial growth.关键词
内源微生物/油藏/脱氧核糖核酸/梯度凝胶变性电泳/激活剂Key words
indigenous microorganisms/ reservoir/ DNA/ DGGE(Denaturing Gradient Gel Electro-phoresis)/ activating nutrients分类
资源环境引用本文复制引用
包木太,刘涛,高光军,郭辽原,李希明..不同激活剂条件下油藏内源微生物激活过程中DGGE分析[J].湖南大学学报（自然科学版）,2009,36(11):67-72,6.基金项目
国家自然科学基金资助项目(50604013) （50604013）
