湖泊科学2009，Vol.21Issue(6)：801-805,5.
太湖梅梁湾水华微囊藻基因型组成和产毒微囊藻丰度的变化
Dynamics of composition of different Microcystis spp. genotypes and abundance of toxic Microcystis in Meiliang Bay of Lake Taihu during bloom
摘要
Abstract
Dynamics of composition of different Microcystis spp. genotypes from June to November, 2008 in Meiliang Bay of Lake Taihu in China were investigated based on denaturing gradient gel electrophoresis analysis of PCR-amplified ITS (internal transcribed spacer sequence) fragments. Simultaneously, we investigated the relative abundance of microeystin-producing Microcystis spp. (the ratio of the toxic Microcystis to total Microcystis) based on microcystin synthetaae gene (mcyA) and partial Microcystis-specific 16S rDNA sequence using real-time PCR. The results of this study indicated that there were shifts of composition of different Microcystis genotypes during algae bloom in Lake Taihu. The most abundant genotypes were found in the late of August, September and October. Genotypes M5 and M10 were found every month. A total of twelve main genotypes were found, and the relative abundaace of each genotype changed during the development of algae bloom. Quantitative real-time PCR (qRT-PCR) revealed that the abundance of toxic Microcystis increased from 0.75% to 32.16% from June to October and decreased significantly in November.关键词
微囊藻/产毒微囊藻/ITS/实时荧光定量PCR/DGGE/水华/太湖Key words
Microcystis/ toxic Microcystis/ ITS/ qRT-PCR/ DGGE/ bloom/ Lake Taihu分类
天文与地球科学引用本文复制引用
施丽梅,蔡元锋,杨华林,李朋富,孔繁翔,孔令东..太湖梅梁湾水华微囊藻基因型组成和产毒微囊藻丰度的变化[J].湖泊科学,2009,21(6):801-805,5.基金项目
国家重点基础研究发展计划(973)项目(2008CB418004)和江苏省自然科学基金项目(BK2007150)联合资助. （973）
