四川大学学报（医学版）2009，Vol.40Issue(6)：1127-1129,3.
荧光染料CFSE在实验性葡萄膜炎T细胞增殖研究中的应用
Tracking T Lymphocytes Proliferation with Vital Dye CFSE in Experimental Autoimmune Uveitis
雷松 1毛咏秋2
作者信息
- 1. 四川大学华西医院,病理科,成都,610041
- 2. 四川大学华西医院,生物治疗国家重点实验室
- 折叠
摘要
Abstract
Objective To track T lymphocytes proliferation with vital dye CFSE in experimental autoimmune uveitis (EAU). Methods B10RIII mice were immunized with synthetic peptide of interphotoreceptor retinal-binding protein (IRBP161-180) to develop EAU. The proliferation of IRBP-specific T cell subsets was detected by CFSE staining, fluorescent antibody labeling, and flow cytometry. Results The IRBP-specific T cells divided after 4 days of stimulation with IRBP161-180, halving the fluorescence intensity. The proliferations of CD4~+ and CD8~+ T cells were asynchronous, with CD8~+ T cells dividing more vigorously and having more drop in percentage of parent cells(P<0. 01). Conclusion CFSE-Labeling can detect the proliferation of autoreactive T cells and their subsets in EAU effectively.
关键词
CFSE/实验性葡萄膜炎/活化T细胞Key words
CFSE /Experimental autoimmune uveitis /Autoreactive T cell分类
医药卫生引用本文复制引用
雷松,毛咏秋..荧光染料CFSE在实验性葡萄膜炎T细胞增殖研究中的应用[J].四川大学学报（医学版）,2009,40(6):1127-1129,3.
