四川大学学报（医学版）2009，Vol.40Issue(6)：978-982,5.
ARLTS1基因在早发性卵巢癌中的突变分析
Polymorphism of ARLTS1 Gene in Early-onset Ovarian Cancer
杨小芸 1余海 2彭芝兰1
作者信息
- 1. 四川大学华西第二医院,妇产科,成都,610041
- 2. 四川大学华西医院,麻醉科
- 折叠
摘要
Abstract
Objective To evaluate the impact of ARLTS1 gene mutations among Chinese Han-nationality patients with early-onset epithelial ovarian carcinoma (OC) in Southern China. Methods Tumor and blood samples collected from early-onset, late-onset epithelial ovarian carcinoma patients and control group were collected. After purification of DNA, the ARLTS1 gene fragments were amplified with PCR. The DHPLC was applied to analyze the PCR products. The mutation products were confirmed with DNA sequencing analysis. Results ARLTS1 Cysl48Arg revealed a significant association with an increased risk in early-onset OC group compared with both late-onset cases and controls. ARLTS1 Ser99Ser revealed no impact on both early-onset OC group and late-onset cases. Conclusion A significantly increased risk for young ovarian cancer patients with ARLTS1 Cysl48Arg variant were observed, which indicate that ARLTS1 may play a important role in early-onset of OC.关键词
早发性卵巢癌/ARLTS1基因/变性高效液相色谱法(DHPLC)/DNA直接测序Key words
Early-onset ovarian cancer/ARLTS1 gene/DHPLC/DNA direct sequencing分类
医药卫生引用本文复制引用
杨小芸,余海,彭芝兰..ARLTS1基因在早发性卵巢癌中的突变分析[J].四川大学学报（医学版）,2009,40(6):978-982,5.
