四川大学学报（医学版）2009，Vol.40Issue(6)：994-999,6.
苦参碱对肺成纤维细胞TGF-β_1信号转导途径的干预作用
The Effect of Matrine on the Signal Transduction Pathway of Transforming Growth Factor-β_1 in Human Lung Fibroblast Cells
摘要
Abstract
Objective To investigate the effect of matrine on the signal transduction pathway of transforming growth factor-pi (TGF-β_1). Methods The effect of TGF-β_1, Staurosporine, PD98059 and matrine on the expression of CTGF. CTGF mRNA, p-Smad2, and p-ERK_(1/2) were evaluated by Western blot in cultured HLF-02 cells. Immunohistochemical staining was applied to test the expression of CTGF and the expression level of CTGF mRNA was assayed by RT-PCR. Results Basal levels of CTGF protein were observed in cultured HLF-02. The expression of CTGF, CTGF mRNA, p-Smad2 and p-ERK_(1/2) in HLF-02 cells were stimulated significantly (P< 0. 01) by 1 ng/mL TGF-β_1 treatment. The increase of CTGF protein was inhibited significantly by pre-incubation with Staurosporine (SP) and PD98059 (P<0. 01). And matrine could markedly inhibited the up-regulation of CTGF, CTGF mRNA, p-Smad2 and p-ERK_(1/2) (P<0. 05or P<0. 01) in a concentration-dependant manner. Conclusion CTGF, CTGF mRNA, p-Smad2 and p-ERK_(1/2) of HLF-02 cells could be significantly up-regulated after treated with TGF-β1, and these increases could be inhibited significantly by matrine. This implied that matrine inhibit the expression of CTGF and CTGF mRNA induced by TGF-β_1 through the Smad2 and ERK signal transduction pathways.关键词
人肺成纤维细胞/转化生长因子-β_1/结缔组织生长因子/苦参碱Key words
Human lung fibroblasts /Transforming growth factor-β_1/Connective tissue growth factor /Matrine分类
医药卫生引用本文复制引用
凌伟,谢敏,石俊青..苦参碱对肺成纤维细胞TGF-β_1信号转导途径的干预作用[J].四川大学学报（医学版）,2009,40(6):994-999,6.基金项目
四川省科技厅攻关项目(0040205301259)资助 （0040205301259）
