四川大学学报（医学版）2009，Vol.40Issue(6)：1000-1002,1037,4.
3,4-亚甲基二氧基甲基苯丙胺诱导大鼠神经元凋亡及凋亡相关因子的表达
Neuron Apoptosis Induced by 3,4-methylenedioxy Methamphetamine and Expression of Apoptosis-related Factors in Rat Brain
摘要
Abstract
Objective To study the neuron apoptosis induced by I. P 3.4-methylenedioxy methamphetamine (MDMA) and the expression of apoptosis-related factors in rat brain. Methods Twenty rats were divided into 4 groups. In group A, the rats were injected intraperitoneally with single dosage of saline, while the rats of group B, C, D were injected I. P with MDMA in different regimen, which were 20 mg/kg, single injection in group B, 20 mg/ kg twice a day (8 am and 8 pm) , for 2 day in group C, as well as 20 mg/kg, twice a day (8 am and 8 pm) for 4 days. Neuron apoptosis were measured by TUNEL, and the expression of Caspase-3 and CytC were detected by immunohistochemistry. Results Compared with saline group, apoptosis neurons were detected at the related brain regions (such as frontal cortex, hippocampus and striatum) of the rats in MDMA treated groups;Expression of Caspase-3 and CytC was observed at different level. Compared with group B, the number of apoptosis neurons of group C and D increased, and also the apoptosis-related factors in the brain tissue increased (P<0. 05). Conclusion MDMA could induce neurons apoptosis and the expression of apoptosis-related factors such as Caspase-3 and CytC in rat brain.Objective To study the neuron apoptosis induced by I. P 3.4-methylenedioxy methamphetamine (MDMA) and the expression of apoptosis-related factors in rat brain. Methods Twenty rats were divided into 4 groups. In group A, the rats were injected intraperitoneally with single dosage of saline, while the rats of group B, C, D were injected I. P with MDMA in different regimen, which were 20 mg/kg, single injection in group B, 20 mg/ kg twice a day (8 am and 8 pm) , for 2 day in group C, as well as 20 mg/kg, twice a day (8 am and 8 pm) for 4 days. Neuron apoptosis were measured by TUNEL, and the expression of Caspase-3 and CytC were detected by immunohistochemistry. Results Compared with saline group, apoptosis neurons were detected at the related brain regions (such as frontal cortex, hippocampus and striatum) of the rats in MDMA treated groups;Expression of Caspase-3 and CytC was observed at different level. Compared with group B, the number of apoptosis neurons of group C and D increased, and also the apoptosis-related factors in the brain tissue increased (P<0. 05). Conclusion MDMA could induce neurons apoptosis and the expression of apoptosis-related factors such as Caspase-3 and CytC in rat brain.关键词
3,4-亚甲基二氧基甲基苯丙胺/神经元凋亡/Caspase-3/CytCKey words
3,4-methylenedioxy methamphetamine /Neuron apoptosis /Caspase-3 /Cytchrome C分类
医药卫生引用本文复制引用
王雪,祝三平,况伟宏,李静,孙啸,黄明生,孙学礼..3,4-亚甲基二氧基甲基苯丙胺诱导大鼠神经元凋亡及凋亡相关因子的表达[J].四川大学学报（医学版）,2009,40(6):1000-1002,1037,4.基金项目
四川大学华西医院基金(基金编号:134040162)资助 （基金编号:134040162）
