四川大学学报（医学版）2009，Vol.40Issue(6)：1033-1037,5.
苦瓜蛋白RIP的免疫毒性研究
Study on the Immunotoxicity of Ribosome Inhibiting Protein of Momordica Charantia
摘要
Abstract
Objective To separate and purify ribosome inhibiting protein (RIP) from Momordica charantia (bitter melon) seeds and to evaluate its acute toxicity and immunotoxicity in animal. Methods Ion exchange chromatography and gel filtration chromatography were applied in the separating and purifying of RIP from Momordica charantia seeds. Then the acute toxicity testing of RIP in mice was conducted to obtain its half lethal dose (LD_(50)). Active systemic anaphylaxis (ASA) test in guinea pig and passive cutaneous anaphylaxis test (PCA) in rat were performed to evaluate its immunotoxicity. Results The LD_(50) (iv) in mice of RIP was 25. 2 mg/kg in ASA. guinea pigs of the higher and lower RIP group all appeared stong allergic responses and most of them died quickly. In PCA. obvious blue dye in skin were observed in SD rats of the RIP group. Conclusion RIP getting from Momordica charantia seeds had a relatively strong immunotoxicity in animals.关键词
苦瓜核糖体失活蛋白/急性毒性试验/免疫毒性Key words
Ribosome inhibiting protein of momordica charantia /Acute toxicity/Immunotoxicity分类
医药卫生引用本文复制引用
万莉,孟延发,沈富兵,郑崛村,邓念华,张立实..苦瓜蛋白RIP的免疫毒性研究[J].四川大学学报（医学版）,2009,40(6):1033-1037,5.基金项目
国家自然科学基金(批准号30770232)资助 （批准号30770232）
