海洋科学2009，Vol.33Issue(11)：24-31,8.
海鞘血细胞培养和细菌污染控制
Ascidian hemocytes culture and the prevention of bactirail contamination
摘要
Abstract
In this study,ascidian hemocytes were cultured in vitro and the identiffcation and Drevention of bacterial contamination were investigated.After 1 h incubation,hemocytes spread and formed a flat sheet consisting of nine types of cells.Most cultured cells were round and several cells were amebocytes.Round cell types could be maintained for 2 weeks at least in vitro culture,whereas in vitro COHld survived for very short time.Two bacterial strains contaminated in cell culture were isolated from the bacterial contamination samples by culture and purity cuhure.Two bacterial strains were gram-negative bacterium.The detected bacteria were isolated and identified as the members of by 16S rDNA sequencing.Drug sensitivity test detected that Vibrio showed resistance to chloromycetin and andciprofloxacin,Shewanella showed resistance to imipenem and chloromycetin.In order to control the microbial contamination in cell culture,three antibiotics mixtures were compared.It is shown that gentanycin was efective to prevent bacterial contamination.关键词
血细胞培养/细菌污染/16S rDNA/药敏试验/海鞘(Ciona savignyi)Key words
hemocytes culture/bacterial contamination/16S rDNA/drug sensitivity test/ascidian(Ciona savignyi)分类
生物科学引用本文复制引用
张晓明,金松君,相建海..海鞘血细胞培养和细菌污染控制[J].海洋科学,2009,33(11):24-31,8.基金项目
国家863计划项目(2006AA10A401) （2006AA10A401）
