四川大学学报（医学版）2009，Vol.40Issue(6)：1053-1055,3.
M-Enk在猫背根节切除后脊髓Ⅱ板层的表达
M-Enk Expressing in the Lamina Ⅱ of Cat Spinal Cord after Deafferentation CHEN
陈建军 1杨琴1
作者信息
- 1. 成都医学院,解剖学与组织胚胎学教研室,成都,610083
- 折叠
摘要
Abstract
Objective To investigate the Methionine Enkephalin (M-Enk) expression in lamina II of cat spinal cord and the atypical complex terminal (ACT) after complete dorsal rhizotomy. Methods 10 male adult cats were divided into 2 groups after carrying out the complete dorsal rhizotomy: acute group, which survived 2 weeks; chronic group, which survived 2 months. At maturity, all cats were perfused after anaesthesia, the L6 segments of the spinal cord were removed and prepared for light and electron microscopy of M-Enk immunohistochemistry and for observing of ACT. Results M-Enk immuno-reactivity can be seen in laminal II lateral sides both in operation and control sides in acute group. They were both decreased in chronic group on the photos by naked eyes, and little intensive in operation side on the photos. The immuno-positive ACTs have been founded in the Lamina II of chronic operation side beside some M-Enk immunopositive simple terminals, they are round or ellipse in shape and usually form flat or convex two synapse with two post-compounds, ACTs contained both clear vesicles and dense core vesicles. Conclusion M-Enk express in operation side after complete dorsal rhizotomy is mainly on the lateral side in lamina II. The M-Enk immuno-positive ACT may imply the mechanism of spinal cord plasticity.关键词
脑啡肽(M-Enk)/脊髓可塑性/电镜免疫组化/猫Key words
M-Enk/Spinal plasticity /Immunohistochenistry EM /Cat分类
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陈建军,杨琴..M-Enk在猫背根节切除后脊髓Ⅱ板层的表达[J].四川大学学报（医学版）,2009,40(6):1053-1055,3.
