海洋科学2009，Vol.33Issue(11)：32-36,5.
西施舌外套膜蛋白质组双向电泳体系的构建
Establishment of two-dimensional electrophoresis system for proteome of Coelomactra antiquata mantle
摘要
Abstract
Coelomactra antiquata is a high value economic shellfish which is famous treasure feast food and medical care of aquaculture consumption.The border cells of C.antiquata mantle can secrete calcium carbonate to form shells.By comparing the experimental analysis of different pH gradient strips,the optimal conditions of hydration,and different staining methods on the impact of the results of two-dimensional gel electrophoresis,we established the mantle proteome two-dimensional gel electrophoresis system of C.antiquata,which provide the basis to study the protein composition of formation of C.antiquata mantle from the protein level case.The results showed that:using pH 4～7 with the non-linear strip,the inclusion of hydration desalting step,staining method using silver nitrate staining,can obtain the two-dimensional gel electrophoresis map with a high-resolution and high reproducibility which laid the foundation for further research the protein composition of C.antiquata mantle.关键词
双向电泳/蛋白质组学/西施舌(Coelomactra antiquata)/外套膜Key words
two-dimensional electrophoresis/proteomics/Coelomactra antiquate/mantle分类
生物科学引用本文复制引用
田美,申欣,程汉良,孟学平..西施舌外套膜蛋白质组双向电泳体系的构建[J].海洋科学,2009,33(11):32-36,5.基金项目
江苏省自然科学基金项目(BK2007066) （BK2007066）
