海洋科学2009，Vol.33Issue(10)：63-67,5.
栉江珧第1卵裂抑制型雌核发育二倍体人工诱导的研究
Artificial induction of gynogenetic diploids by suppression of the first cleavage in Atrina pectinata
摘要
Abstract
The induction of gynogenesis diploids by suppression of the first cleavage in Atrina pectinata was attempted using the treatment of 6-dimethylaminopurine(60 rag/L; 6-DMAP). The results showed that the treatment of zygotes with 6-DMAP at 60 rain postinsemination for 15 min at water temperature of 24℃ produced more expected D-shaped larvae, and the rate of D-shaped larvae and level of diploids were 14. 7% and 22.7%, respectively. According to cytological observations, 6-DMAP disrupted the spindle at mi-tosis and inhibited chromosome movement, resulting in the formation of one diploid female nucleus. Cyto-logical evidence of the induction of gynogenetic diploids in A. Pectinata was demonstrated.关键词
栉江珧(Atrina pectinata)/第一卵裂抑制型/雌核发育二倍体/6-DMAPKey words
Atrina pectinata/ first cleavage/ gynogenetic diploid/ 6-DMAP分类
生物科学引用本文复制引用
杨青,李琪,于瑞海,孔令锋,郑小东..栉江珧第1卵裂抑制型雌核发育二倍体人工诱导的研究[J].海洋科学,2009,33(10):63-67,5.基金项目
国家自然科学基金(30571442) （30571442）
教育部重大培育项目(707041) （707041）
