解放军医学杂志2009，Vol.34Issue(12)：1398-1402,5.
2009年新型甲型H1N1流感病毒血凝素基因进化及变异特征分析
Genetic evolution and variation of hemagglutinin gene of the novel A/H1N1 influenza pandemic virus in 2009
摘要
Abstract
Objective To elucidate the characteristics of genetic evolution and variation trend of hemagglutinin ( HA). a major antigenic gene of the novel A/H1N1 influenza pandemic virus in 2009. Methods HA gene sequences of the novel A/H1N1 influenza pandemic virus in 2009, as well as that of the A/H1N1 influenza virus spread in North America, Europe and Asia, were downloaded from NCBI database. MEGA4.0 software was used to analyze the constructed phylogenetic tree of the selected sequences of HA gene. The nucleotide homology and amino acid specificity of the novel A/H1N1 influenza pandemic virus in 2009 were analyzed and compared with those in North America, Europe and Asia regions. Results HA gene of the novel A/H1N1 influenza pandemic virus in 2009 showed a highest homology (93. 2%-93. 4%) with the corresponding sequences of A/swine/H1N1 influenza virus isolated from 2006 to 2007 in the United States, with a typical temporal evolution relationship with the A/swine/H1N1 influenza virus isolated from 1930 to 2007 in the United States, while it had no relationship with the A/swine/H1N1 strains isolated from Europe and Asia. There existed significant difference between the HA gene of the novel A/H1N1 strain and the A/human/H1N1 virus and the vaccine strains in nucleotide sequences and the deduced amino acids sequences. No mutations in antigenic site were found since the influenza epidemic occurred throughout the world. Conclusions HA gene of A/H1N1 influenza pandemic virus in 2009 is originated from A/swine/HlNl influenza virus evolved in North America. No apparent mutation is found in this short pandemic period.关键词
流感/人/流感病毒A型/H1N1亚型/基因/HA/抗原变异Key words
influenza/ human/ influenza A/ H1N1 subtype/ genes/ HA/ antigenic variation分类
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李淑华,韩一芳,谢佳新,韩磊,苏彤,鹿文英,曹广文..2009年新型甲型H1N1流感病毒血凝素基因进化及变异特征分析[J].解放军医学杂志,2009,34(12):1398-1402,5.基金项目
军队"十一五"科技攻关计划资助项目(06G65) （06G65）
上海市自然科学基金资助项目(07ZR14141) （07ZR14141）
上海市公共卫生"三年行动计划"重点学科资助项目(08GWZX0201.08GWZX0101) （08GWZX0201.08GWZX0101）
