解放军医学杂志2009，Vol.34Issue(12)：1403-1407,5.
2009年新型甲型H1N1流感病毒聚合酶编码基因的遗传特性及重要功能位点变异分析
Genetic characteristics and variations at important functional sites of polymerase coding genes of the novel A/H1N1 influenza pandemic virus in 2009
摘要
Abstract
Objective To elucidate the hereditary characteristics and variations of important functional domains of polymerase PA, PB1 and PB2 gene of the novel A/H1N1 influenza pandemic virus in 2009. Methods The sequences of PA, PB1 and PB2 gene of the novel A/H1N1 strains, and the reference sequences according to the years, locations, hosts and subtypes of influenza virus were retrieved from NCBI database. All the sequences were contrasted with MEGA4.0 software. The phylogenetic tree was constructed with the neighbor-joining method. All the deduced PB2 protein sequences were compared among the influenza strains, the avian influenza virus and the human A/H1N1 influenza virus found in different years. Results The sequence homology exceeded 99. 8% of PA, PB1 and PB2 gene of the novel A/H1N1 influenza virus isolated from different locations and different time. The sequences of PA, PB1 and PB2 gene isolated from different locations and time showed a high homology, clustered in a unique new clade, and close to avain influenza virus. Phylogenesis indicated that all the PA, PB1 and PB2 gene originally evolved from avain influenza virus. Alignments of the deduced protein sequences showed that the 627th amino acid of the PB2 gene of novel A/H1N1 strains was glutamic acid (Glu) , which was the same as that of avian influenza virus, rather than human H1N1 virus. Conclusions The novel A/H1N1 influenza virus from the same origin leads to the outbreak in 2009, and it is of atypical high pathogenicity. The polymerase gene of avian influenza virus might partially reassort with the novel A/H1N1 virus.关键词
流感/人/流感病毒A型/H1N1亚型/基因/聚合酶/遗传/变异(遗传学)Key words
influenza/ human/ influenza A/ H1N1 subtype/ genes/ pol/ heredity/ variation (genetics)分类
医药卫生引用本文复制引用
韩磊,谢佳新,殷建华,韩一芳,鹿文英,曹广文..2009年新型甲型H1N1流感病毒聚合酶编码基因的遗传特性及重要功能位点变异分析[J].解放军医学杂志,2009,34(12):1403-1407,5.基金项目
军队"十一五"科技攻关计划资助项目(06G65) （06G65）
上海市自然科学基金资助项目(07ZR14141) （07ZR14141）
上海市公共卫生"三年行动计划"重点学科资助项目(08GWZX0201,08GWZX0101) （08GWZX0201,08GWZX0101）
