吉林大学学报（理学版）2009，Vol.47Issue(6)：1313-1317,5.
紫外光谱在线监控动态微波辅助萃取半枝莲中的野黄芩苷
On-line Monitoring of Dynamic Microwave-assisted Extraction of Scutellarin from Scutellaria barbata by UV Spectroscopy
摘要
Abstract
A dynamic microwave-assisted extraction ( DMAE) approach was established for the extraction of scutellarin from Scutellaria barbata. The overall extraction process was monitored by on-line measuring the UV absorbance of the extract. The extraction conditions were optimized using orthogonal experimental design. The content of scutellarin was off-line analyzed by HPLC. The detection and quantification limits of scutellarin obtained were 0.28 mg/L and 0.93 mg/L, respectively. The within-day and between-day precisions (RSD) were 1. 7% and 3. 6% , respectively. The mean recovery of scutellarin was 98. 3%. The DMAE was compared with Soxhlet extraction and pressurized MAE ( PMAE) respectively. The results show that the dynamic method is superior to PMAE in preventing analyte degradation, and also to soxhlet extraction in the reduction of time and solvent required. It is more important that the extraction process can be visually observed by UV on-line monitoring.关键词
在线监控/动态微波辅助萃取/紫外光谱/半枝莲/野黄芩苷Key words
on-line monitoring/ dynamic microwave-assisted extraction/ UV spectroscopy/ Scutellaria barbata/ scutellarin分类
化学化工引用本文复制引用
金海燕,陈立钢,周秀清,张锁秦,刘庆文..紫外光谱在线监控动态微波辅助萃取半枝莲中的野黄芩苷[J].吉林大学学报（理学版）,2009,47(6):1313-1317,5.基金项目
吉林省科技发展计划项目基金(批准号: 20050560). （批准号: 20050560）
