吉林农业大学学报2009，Vol.31Issue(6)：759-762,4.
猪圆环病毒Ⅱ型ORF2部分基因的克隆及原核表达
Cloning and Prokaryotic Expression of ORF2 Gene of Porcine Circovirus Type 2
摘要
Abstract
Based on the published nucleotide sequence of ORF2 gene of porcine circovirus 2, a pair of primers were designed and synthesized. The 579 bp was amplified by polymerase chain reaction(PCR) from Jilin strain, and then the fragment was cloned into pET-32a vector, BamHI and Hind HI restriction endonuclease analysis and DNA sequencing were used to identify the recombinant plasmid of pET-32a-ORF2. A recombinant vector was transformed into E. Coli BL21 cells. Then about 40kD fusion protein was expressed in recombinant strain BL21 after being induced by IPTG.The expressed protein confirmed by SDS - PAGE and Western blotting analysis of reactionogenicity can react with the polyclonal antibody against PCV2.关键词
猪圆环病毒Ⅱ型/ORF2/克隆/原核表达Key words
porcine circovirus type 2/ ORF2 gene/ cloning/ prokaryotic expression分类
农业科技引用本文复制引用
王伟利,边少国,肖成蕊,孟庆峰,刘和平..猪圆环病毒Ⅱ型ORF2部分基因的克隆及原核表达[J].吉林农业大学学报,2009,31(6):759-762,4.基金项目
国家质检总局项目(2005IK042) （2005IK042）
