吉林农业大学学报2009，Vol.31Issue(6)：771-776,6.
DNA不同提取方法对养殖池塘底泥细菌多样性PCR-DGGE检测的影响
Influence of Different DNA Extraction Methods on Microbial Diversity PCR-DGGE Detection of Culture Pond Sendiment
摘要
Abstract
In this research, PCR - DGGE technique was used for studying five different extraction methods of microbial DNA of the pond sediment in difference of microbial diversities. The purpose is to try to find extraction methods of DNA which can be best used to objectively reflect the diversities and advantages of the bacteria of the pond sediment, and to evaluate comprehensively the five extraction methods. The results showed that: DNA in the crude extract of two humic acids and protein purity is of no positive correlation; the crude DNA extract of the five extraction methods, after purification, yields good results with PCR; broken composite method is used in the extraction of microbial DNA in the sediment ponds, helping enhance microbial diversity; DNA extraction method is the key factor of pond sediment microbial diversity by DGGE, and the diversity has poor relationship with the output and size of DNA; the similarity of the five ways is as high as 0.79, so it can be substituted for one another to conduct a rough analysis of mierobia] diversity; the improved Martin-Laurent Act is a better DNA extraction method for PCR -DGGE microbial diversity study in sediment ponds.关键词
养殖池塘底泥/细菌多样性/DNA提取/PCR-DGGE/优势种群Key words
culture pond sediment/ microbial diversity/ DNA extraction/ polymerase chain reaction-denaturing gradient gel electrophoresis(PCR - DGGE) / dominant species分类
农业科技引用本文复制引用
王宇,林文辉,王亚军,毕晔,石存斌,吴淑勤..DNA不同提取方法对养殖池塘底泥细菌多样性PCR-DGGE检测的影响[J].吉林农业大学学报,2009,31(6):771-776,6.基金项目
国家科技支撑计划项目(2006BAD03B05),广东省科技计划项目(2006B20301028) （2006BAD03B05）
