解剖学杂志2009，Vol.32Issue(5)：599-602,4.
胎肝源性细胞促进人骨髓间充质干细胞向肝细胞的定向分化
Fetal liver-derived cells stimulate differentiation of human mesenchymal stem cells into hepatocytes in vitro
摘要
Abstract
Objective:To investigate effects of human fetal liver-derived cell (HFLC) on differentiation of human mesenchy-mal stem cells (MSCs) into hepatocytes. Methods:Fetal hepatocytes from medical abortion and adult human MSCs labeled with Hoechst 33342 were co-cultured directly and/or separately in a dual chamber dish. Cell morphology was observed under a phase microscope. Hepatocyte specific markers alpha-fetoprotein (AFP), cytokeratin (CK)18 and CK19 were identified by immunofluoresent staining and glycogen storage was detected by Periodic Acid-Schiff (PAS) staining at different time intervals. Results: In the direct co-culture system, immunofluorescent staining demonstrated that the differentiated cells had the ability of expressing AFP, CK18 and CK19, and increased with time of differentiation. In addition, the differentiated cells had functional properties of hepatocytes, such as glycogen storage, which was evaluated by PAS staining. This phenomenon did not appear when HFLC were separated from human MSCs in a dual chamber dish. Conclusion:The direct co-culture of HFLC with adult human MSCs may create a particularly favorable microenvironment for the transdifferentiation of the latter into hepatocyte-like cell, which may serve as a cell source for cell therapy of hepatic tissues.关键词
骨髓间充质干细胞/胎肝源性细胞/共培养/肝细胞/分化Key words
mesenchymal stem cell/fetal liver-derived cell/co-culture/hepatocyte/differentiation分类
医药卫生引用本文复制引用
汪泱,车媛梅,张一,谢安,崔苏萍,娄远蕾..胎肝源性细胞促进人骨髓间充质干细胞向肝细胞的定向分化[J].解剖学杂志,2009,32(5):599-602,4.基金项目
江西省科技厅重点项目 （）
江西省自然科学基金(2007GZY1470) （2007GZY1470）
