腺病毒介导胞嘧啶脱氨酶基因联合黄连素对直肠癌细胞的杀伤作用OA北大核心CSCDCSTPCD
Adenoviral mediated cytosine deaminase gene combining with berberine in killing rectal cancer cells
目的:探讨黄连素联合腺病毒介导的胞嘧啶脱氨酶自杀基因(Ad-CD)对直肠癌细胞的体外杀伤作用.方法:用重组腺病毒介导外源CD基因转染到人直肠癌细胞株HR-8348,检测病毒的转导效率和CD基因表达.用四甲基偶氮唑盐(MTT法)检测黄连素联合Ad-CD基因对HR-8348细胞存活率及体外旁观者效应的影响.结果:在250μg/ml 5-氟胞嘧啶(5-FC)浓度下,0 1、 0 3、 3 0、 30 0μmol/L浓度的黄连素联合5-FC对直肠癌…查看全部>>
Objective:To evaluate antitumor effect of berberine and adenovirus-mediated cytosine deaminase (CD) suicide gene on rectal cancer cells in vitro. Methods: Mediated by adenovirus, the CD gene was transfected into HR-8348 rectal cancer cell line. After identification of transfection rate and CD expression, the cell viability and "by-stander effect" were examined by using MTT assay. Results:Based on 250 μg/ml 5-FC, 0.1, 0. 3, 3. 0, 30. 0 μmol/l berberine produc…查看全部>>
刘曾旭;余庆;马学强;刘德明;朱雄文
南昌大学基础医学院解剖学教研室,南昌330006南昌大学图书馆,南昌330031浙江省台州市立医院,台州318000南昌大学基础医学院解剖学教研室,南昌330006浙江省台州市立医院,台州318000
医药卫生
直肠癌细胞胞嘧啶脱氨酶黄连素基因疗法
rectal cancer cellcytosine deaminaseberberinegene therapy
《解剖学杂志》 2009 (5)
635-637,646,4
浙江省医药卫生科技计划(20068145)
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