解剖学杂志2009，Vol.32Issue(5)：651-653,659,4.
创伤后应激障碍大鼠海马细胞凋亡中细胞色素C的表达与释放
Expression and release of cytochrome C in cellular apoptosis of hippocampus in rat model of posttraumatic stress disorder
摘要
Abstract
Objective:To observe changes of apoptosis and cytochrome C expression and release in hippocampus of rat model of posttraumatic stress disorder (PTSD), and further explore their relationship. Methods:Single prolonged stress (SPS) was used to duplicate a rat model of PSTD. The rats undergoing SPS were analyzed at day 4, 7, 14 and 28 by using TUNEL for apoptosis of hippocampal neurons, immunohistochemical staining and Western blot for expression of cytochrome C, and electron microscopic enzyme histochemistry for release of cytochrome C in mitochondria. Results: Four days after SPS, the cytochrome C was released from mitochondria to cytoplasm and its expression was up to peak until the 7~(th) day, when the expression of cytochrome C decreased gradually. Apoptosis in hippocampus was peaked at day 7. Conclusion:Cytochrome C may play an important regulatory role in cellular apoptosis in the rat hippocampus of PTSD.关键词
创伤后应激障碍/细胞色素C/凋亡/海马/大鼠Key words
posttraumatic stress disorder/cytochrome C/apoptosis/hippocampus/rat分类
医药卫生引用本文复制引用
李晓明,韩芳,石玉秀..创伤后应激障碍大鼠海马细胞凋亡中细胞色素C的表达与释放[J].解剖学杂志,2009,32(5):651-653,659,4.基金项目
国家自然科学基金(30600341) （30600341）
