解剖学杂志2009，Vol.32Issue(5)：660-662,3.
大鼠脊髓星形胶质细胞培养方法的改良
A modified method to culture astrocytes from neonatal rat spinal cord
摘要
Abstract
Objective:The culture method of astrocytes from spinal cord tissues was modified to provide a more valuable experimental model for research. Methods?Under a sterile condition, the spines of 1-2-day-old rats were dissected to subsequently obtain spinal cord by washing with D-Hank's. Spinal cord cells were dissociated by blowing and then seeded in culture flask. After purified with differential velocity adherent technique, the astrocytes cultured in the second passage was I-dentified with immunofluorescence staining against glial fibrillary acidic protein (GFAP). Results?Astrocytes growed in a good condition through culture; after a second subculture, astrocytes were purified to 99%. Conclusion? Modified technique for astrocytes culture described in present study is feasible due to its easy operation and harvest of astrocyte with effective purity, which provide an effective tool for sequential function research of astrocytes in spinal cord diseases.关键词
脊髓/星形胶质细胞/细胞培养/大鼠Key words
spinal cord/astrocyte/cell culture/rat分类
医药卫生引用本文复制引用
詹燕,渠文生,田代实,王伟..大鼠脊髓星形胶质细胞培养方法的改良[J].解剖学杂志,2009,32(5):660-662,3.基金项目
国家自然科学基金重点项目(30800340,30230140) （30800340,30230140）
