吉首大学学报（自然科学版）2009，Vol.30Issue(5)：87-92,6.
喜树叶中喜树碱和10-羟基喜树碱的HPLC分析
HPLC Analysis of Camptothecin and 10-Hydroxycamptothecin in Camptotheca Acuminata Decne Leaves
摘要
Abstract
An HPLC method for the simultaneous determination of CPT and HCPT in Camptotheca acuminata Decne.leaves was established.Chromatography was performed with a sinochrom ODS column,monitored at 266 am and the mobile phase was gradient H2O/CH3 OH.The solvent gradient program was as follows:methanol-water increased linearly from 40% to 50% at the first 6 min and retained for 2 min with 50%,then increased linearly to 70% in the next 32 min.The average recoveries of CPT in three levels were 100.02%,98.94% and 99.25%,The average recoveries of HCPT were 99.13%,99.66% and 98.97%.Using this assay,CPT and HCPT in leaves of Camptotheca acuminata Decne.in different seasons were determined.The results showed that the accumulation of CPT and HCPT presented evident relativity in the different growth stages.关键词
高效液相色谱法/喜树/叶片/喜树碱/10-羟基喜树碱Key words
HPLC/camptotheca acuminata Decne./leaves/camptothecin(CPT)/10-hydroxy-camptothecin(HCPT)分类
医药卫生引用本文复制引用
李香莉,肖凯军,胡先运,罗心毅,郭祀远..喜树叶中喜树碱和10-羟基喜树碱的HPLC分析[J].吉首大学学报（自然科学版）,2009,30(5):87-92,6.基金项目
Supported by Sino-French Advanced Research Project(PRA B02-07) （PRA B02-07）
Scientific and technological achievements promotion project of Guangzhou city(2006C13G0011) （2006C13G0011）
