江西农业大学学报2009，Vol.31Issue(5)：906-910,5.
红叶石楠组培苗玻璃化影响因子及其克服技术研究
Factors in Photinia fraseri Vitrification in Vitro and Its Control
摘要
Abstract
Factors in red tip Photinia (Photinia fraseri) vitrification in vitro were studied. The results showed that rich mass elements of mineral nutrients or high dosage of 6 - BA in the media increased the rate of vitrified plantlets, lower temperature or higher concentration of gelling agents reduced the rate compared with normal culture condition. More vitrified plantlets were found in the media solidified by carrageenan than in the media by agar in the similar gelling degree. The vitrification rate went down with the increase of multiplication generations. The plantlets with some leaves vitrified could be de - vitrified at 61. 67% ,57. 92% and 47. 08% respectively, treated in the way as follows for 60 days: 1. culture temperature changed from 25℃ all daytime to 25℃ for 16 hours in light period and 20℃ for 8 hours in dark period, 2. concentration of 6 - BA decreased from 1.5 mg/L to 0.5 mg/L, 3. the quantity of solidifying agent Carageenan added to the media increased from 7.0 g/L to 8.0 g/L, but the shoots vitrified completely had no change.关键词
红叶石楠/组织培养/玻璃化/控制Key words
red tip photinia/tissue culture/vitrification/control分类
农业科技引用本文复制引用
杨雪,吴国盛,范加勤..红叶石楠组培苗玻璃化影响因子及其克服技术研究[J].江西农业大学学报,2009,31(5):906-910,5.基金项目
江苏省科技厅科技兴农项目(BC2001357)和南京市科技局科技兴农项目(2002200) （BC2001357）
