江西农业大学学报2009，Vol.31Issue(5)：915-918,4.
新干特早柚GPAT基因5'端克隆及序列分析
Cloning and Sequencing Analysis of the 5' Fragment of GPAT Gene from Citrus grandis cv. Xingantezaoyou
摘要
Abstract
Citrus maxima cv. Xingantezaoyou' s GPAT gene of 5' end fragment was isolated from the leaf by means of 5 ' - RACE technology. The cDNA fragment is 832 bp long ( GenBank Accession No: DQ193970). The nucleotide sequence analysis showed that these sequences of GPAT 5' end fragment are greatly different from those of other plants. The best nucleotide similarity to Citrus unshiu was 98.9% but those Carthamus tinctorius, Arabidopsis thaliana ,Elaeis guineensis, Pisum sativum and Viciafaba were 62.5% ,54.6% , 48.2% ,47.4% and 46.9% respectively. The deduce amino acids sequence analysis showed the similarity to Carthamus tinctorius,Arabidopsis thaliana, Elaeis guineensis,Pisum sativum and Capsicum annuum were 51.6% , 60% ,45.1% ,48% and 52.3%. The amino acid sequences of GPAT gene has genera specificity because the similarity of amino acid sequences to Bacillus amyloliquefaciens is only 18.9%.关键词
新干特早柚/GPAT/RACE/基因克隆/序列分析Key words
Citrus grandis cv. Xingantezaoyou/GPAT/RACE/gene cloning/sequence analysis分类
生物科学引用本文复制引用
吴波,刘勇..新干特早柚GPAT基因5'端克隆及序列分析[J].江西农业大学学报,2009,31(5):915-918,4.基金项目
国家自然科学基金资助项目(30070528)和江西农业大学博士启动基金项目(1791) （30070528）
