昆虫学报2009，Vol.52Issue(11)：1266-1272,7.
基于rDNA ITSl和ITS2序列的褐飞虱、白背飞虱和灰飞虱的分子鉴定
Molecular identification of Nilaparvata lugens (Stal),Sogatella furcifera (Horvath)and Laodelphax striatellus (Fallén) (Homoptera:Deiphacidae) based on rDNA ITS 1 and ITS2 sequences
摘要
Abstract
The complete sequences of rDNA ITS1 and ITS2 were determined for the brown planthopper Nilaparvata lugens, the white-backed planthopper Sogatella fiiTcifera and the small brown planthopper Laodelphax striatellus in order to explore the molecular identification method for them. The flanking regions of rDNA-ITSl and ITS2 of the three planthoppers showed only limited variation, but the sequences of rDNA-ITS1 and ITS2 differed significantly. There are 294 variable sites in the 438 analyzed sites for the ITS1 region, and 177 variable sites in the 403 analyzed sites for the ITS2 region. Species-specific primers of N. lugens, S. furcifera, and L striatellus were designed based on their rDNA-ITSl and ITS2 sequences. The results of PCR amplification of rDNA-ITSl in the three species indicated that the species-specific primers were not applicable. However, the species-specific primers based on the rDNA-ITS2 sequences proved to be useful diagnostic primers for the three planthoppers. It is so concluded that molecular identification of N. lugens, S. furcifera and L striatellus using the species-specific primers from the rDNA-ITS2 region is feasible.关键词
褐飞虱/白背飞虱/灰飞虱/rDNA/ITS1/ITS2/特异引物/分子鉴定Key words
Nilaparvata lugens/ Sogatella furcifera/ Laodelphax striatellus/ rDNA/ ITS1/ ITS2/ diagnostic primers/ molecular identification分类
生物科学引用本文复制引用
刘玉娣,林克剑,韩兰芝,侯茂林..基于rDNA ITSl和ITS2序列的褐飞虱、白背飞虱和灰飞虱的分子鉴定[J].昆虫学报,2009,52(11):1266-1272,7.基金项目
公益性行业(农业)科研专项《水稻褐飞虱综合防控技术研究)(200803003) （农业）
转基因生物新品种培育重大专项《转基因水稻环境安全评价技术》(2008ZX08011-001)和《抗虫转基因水稻对稻飞虱种群影响的环境安全性评价技术》(2009ZX08011-009B) （2008ZX08011-001）
