林产化学与工业2009，Vol.29Issue(5)：109-114,6.
响应面法优化松茸菌丝体多糖超声波提取工艺及其抗氧化研究
Optimization of Ultrasound-assisted Extraction of Tricholoma matsutake ycelium Polysaccharide Using Response Surface Methology and ts Antioxidative Effect
摘要
Abstract
Response surface analysis methodology (RSM) was used for optimizing ultrasound-assisted extraction of Tricholoma matsutake mycelium polysaccharides.Based on single-factor experiments,three independent variables,namely extraction time,water-solid ratio and ultrasonic power,were selected as casual factors.The experiments were arranged according to Box-Behnken central composite experiment design.Response surface analysis was applied to determine the effect of central composite experiment design.Response surface analysis was applied to determine the effect of casual factors on the yield of T.matsutake polysaccharides.The results showed that the optimum ultrasound-assisted extraction condition for T.matsutake polysaccharides was as follows ;T.mastutake 0.1 g,extraction time 223 s,ultrasonic power 427 W and ratio of water to solid 69.7:1 (mL:g).The predicted best extraction yield was 14.52 %,while actual extraction yield was 14.33 %,with relative enor 1.29 %,and the yield was 16.29 % by extracting twice.Anti-oxidative effect of T.matsutake polysaccharides was studied.The result showed that inhibition rates of polysaccharide on ·O_2~- and ·OH could reach 19.32 % and 88.24 %,respectively.关键词
响应面法/超声波提取/松茸菌丝体多糖/抗氧化性Key words
response surface analysis methodology/ultrasound-assisted extraction/Tricholoma matsutake mycelium polysaccharides/anti-oxidative effect分类
化学化工引用本文复制引用
王迪,王伟,牟洪举,王娟,滕利荣,张益波,刘鹤松..响应面法优化松茸菌丝体多糖超声波提取工艺及其抗氧化研究[J].林产化学与工业,2009,29(5):109-114,6.基金项目
中国医学基金会新药发展基金资助项目(20061108) （20061108）
