林业科学2009,Vol.45Issue(10):39-43,5.
外源一氧化氮对鹅毛竹离体叶片衰老的影响
Influence of Exogenous Nitric Oxide on Senescence in Detached Leaves of Shibataea chinensis
摘要
Abstract
We examined effects of exogenous nitric oxide derived from a donor of 10 ~ 50 mg·L~(-1) sodium nitroprusside (SNP) on detached leaves of Shibataea chinensis after anthesis.The results showed that: decreases of leaf chlorophyll and protein content were alleviated to different degree when the isolated leaf was pretreated with different concentrations of SNP solution, among which the 30 mg·L~(-1) SNP had the best effect. Compared to control, the detached leaf SOD and POD activity were respectively decreased by 26.2% and 35.6% , maximum fluorescence of dark adaptation ( F_m) , PS II maximal photochemical efficiency (F_v/F_m), and PS II potential activity (F_v/F_o) were respectively decreased by 42.1 % ,38.2% ,77.0% , but the minimum fluorescence of dark adaptation( F_o) was increased 89.5% after 5 d treatment. However, after the pretreatment with 30 mg·L~(-1) SNP, the changes of all the above physiological parameters were inhibited significantly. The results demonstrated that proper concentration of exogenous nitric oxide was able to slow down efficiently the senescence process of detached leaves of S. Chinensis after anthesis.关键词
NO/鹅毛竹/离体叶片/衰老/叶绿素荧光Key words
nitric oxide/ Shibataea chinensis/detached leaf/senescence/chlorophyll fluorescence分类
农业科技引用本文复制引用
谢寅峰,姚晓华,王林,杨阳,张春霞..外源一氧化氮对鹅毛竹离体叶片衰老的影响[J].林业科学,2009,45(10):39-43,5.基金项目
国家自然科学基金项目(39900115)、江苏省高校自然科学基金项目(05KJB220053)和南京林业大学"十五"人才培养对象基金(PG2003-02)资助. (39900115)