棉花学报2009,Vol.21Issue(6):468-473,6.
比克氏棉及其杂种后代的RAPD分析
RAPD Analysis of Gossypium bickii and Its Hybrid Descendents
摘要
Abstract
The interspecific hybrids were derived from [(Gossypium arboreum×Gossypium bickii) × Gossypium hirsutum], (G. arboreum×G.bickii)×Gossypium barbadense], [(G. arboreum×G. bickii) × (G.hirsutum×Gossypium mustelinum)], (G. arboreum×G.bickii) × (G. hirsutum×Gossypium tomentosum)] after doubling F_1 of (G. arboreum×G.bickii). These hybrids and their parents were analyzed by RAPD. The results showed that: (1)There were 127 amplified DNA bands from 15 primers, 102 bands (80.3 %) were polymorphic; (2) Parents bands and specific bands of hybrids were detected in hybrids; (3) The cluster analysis of materials was studied by group-average method from SAS software. Cluster analysis revealed that these materials were divided into 3 groups. All the results verified the authenticity of hybrids.关键词
比克氏棉/杂种后代/RAPDKey words
Gossypium bickii/hybrid descendents/RAPD分类
农业科技引用本文复制引用
渠云芳,黄晋玲,仪治本,李炳林..比克氏棉及其杂种后代的RAPD分析[J].棉花学报,2009,21(6):468-473,6.基金项目
山西省科技攻关项目(20080311001-1),山西农业大学科技创新基金(2004082) (20080311001-1)