棉花学报2009，Vol.21Issue(6)：503-507,5.
利用PLFA方法研究转Bt基因棉花对土壤微生物群落结构变化的影响
Soil Microbial Community Structure Change Analysis of Transgenic Bt Cotton Based on Phospholipid Fatty Acid Method
摘要
Abstract
The experiment was conducted to evaluate the effects of transgenic Bt cotton on soil microbial community in 2006 under the field based on phospholipid fatty acid (PFLA) method.Three transgenic Bt cotton lines ( JM26,JM44 and CCRI41) and one non-Bt cotton line were used.The results showed transgenic Bt cotton increased amount of PFLA for soil microbe.Bt-cotton soils were characterized by the predominance of actinomycetes,fungi and eukaryotes in comparison with non-Bt cotton soil.In contrast,the microbial community was characterized by the predominance of Gram-positive bacteria in non-Bt cotton soil.Soil microbial community were characterized by the predominance of protoza and Gram-negative bacteria for Bt-cotton soils in square growth stage,and for non-Bt cotton soil in the open boll stage.PFLA anlysis was accepted as indicator for risk evaluation of transgenic Bt cotton on soil microbial community.关键词
转Bt基因棉花/土壤微生物/群落结构/磷脂脂肪酸Key words
transgenic Bt cotton/ soil microbe/ community structure/PLFA分类
农业科技引用本文复制引用
李永山,范巧兰,陈耕,柴永峰,张冬梅,李燕娥..利用PLFA方法研究转Bt基因棉花对土壤微生物群落结构变化的影响[J].棉花学报,2009,21(6):503-507,5.基金项目
国家自然基金(30871601),山西省自然科学基金(2007011092)和山西省农业科学院博士基金(YBSJJ0602) （30871601）
