棉花学报2009，Vol.21Issue(6)：520-523,4.
陆地棉GAI基因原核表达与多克隆抗体制备
Expression of a GAI Gene of Cotton (Gossypium hirsutum) and Preparation of Its Antibody
摘要
Abstract
DELLA protein is the key negative regulator of GA signal response. In order to study the effect of GA on development of cotton fiber, we cloned GhGAI gene, a member of DELLA gene family from the upland cotton EST database by electronic cloning method based on EST. According to the electronic cloning sequence, an integrate coding region was amplified from cotton(Gossypium hirsutum) ovule cDNA. A protein band with molecular weight (Mr) of 62000 was obtained from the prokaryotic expression products by SDS-PAGE. The protein was then purified by His-tag affinity chromatography and used for mutiple-cloned antibody preparation by rabbit immunization. Indirect ELISA test and the Western blot analysis showed that the anti-serum was in high immunocompetence and high specificity.关键词
陆地棉/GhGAI基因/多克隆抗体/蛋白质印迹检测Key words
Gossypium hirsutum/ GhGAI gene/ polyclonal antibody/ Western blot分类
农业科技引用本文复制引用
郑银英,崔百明,祝建波,廖文斌,张根发,彭明..陆地棉GAI基因原核表达与多克隆抗体制备[J].棉花学报,2009,21(6):520-523,4.基金项目
国家973计划资助项目(2004CB117302),国家自然科学基金资助项目(30760099) （2004CB117302）
