南京农业大学学报2009，Vol.32Issue(4)：138-142,5.
高效液相色谱同时检测鱼肉组织中4种磺胺与甲氧苄啶的残留量
Simultaneous determination of 4 sulfonamides and trimethoprim residues in fish tissues by HPLC
摘要
Abstract
The simultaneous determination of sulfonamides and trimethoprim residues in freshwater fishes by HPLC was established. Drugs in samples were extracted with acetonitrile and defatted by acetonitrile-saturated n-hexane, and then the suptants was cleaned up with a HLB column and eluted with methanol and evaporated. The residues was dissolved in the mobile phase. The results indicated that the working curves were linear (r=0. 999) in the range of 0. 025 -1. 0 μg· mL~(-1) for the 4 sulfonamides and 0. 05 -2. 0 μg · mL~(-1) for trimethoprim. When spiked with 0. 02, 0. 10, 0. 20 mg · kg~(-1) of 4 sulfonamides and 0. 04, 0. 20, 0. 40 mg · kg~(-1) of trimethoprim, the average recoveries of samples ranged from 75. 2% to 97. 9% and the RSD (n = 6) were 2. 8%-10. 0% . The limit of detection ( LOD) as estimated by 3 times signal to noise method for sulfadiazine, sulfamerazine, sulfadimidine, sulfame-thoxazole, trimethoprim was 0. 006, 0.007, 0.008, 0. 008 and 0. 019 mg ·kg~(-1), respectively.关键词
磺胺嘧啶/磺胺甲基嘧啶/磺胺二甲基嘧啶/磺胺甲恶唑/甲氧苄啶/罗非鱼/残留/高效液相色谱法Key words
sulfadiazine/sulfamerazine/sulfadimidine/sulfamethoxazole/trimethoprim/tilapia/residue/HPLC分类
化学化工引用本文复制引用
朱晓华,翁棋兰,王静,刘畅,吴光红..高效液相色谱同时检测鱼肉组织中4种磺胺与甲氧苄啶的残留量[J].南京农业大学学报,2009,32(4):138-142,5.基金项目
江苏省科技公共服务平台项目(BM2008158) （BM2008158）
