南京农业大学学报2009，Vol.32Issue(4)：143-147,5.
一株脱氧雪腐镰刀菌烯醇转化菌的分离、鉴定及其转化效果研究
Isolation and identification of a strain of Aspergillus tubingensis with deoxynivalenol biotransformation capability
摘要
Abstract
A colony was isolated from soil by inorganic salt culture media added deoxynivalenol (DON) and numbered NJA - 1. Bt2a and Bt2b were used as primers to amplify the β-tubulin gene of NJA -1. The sequence analysis of Bt2a-Bt2b was submitted to GenBank. The accession number in Gen Bank was DQ902579. Sequence analysis and morphology observation indicated that NJA-1 belonged to Aspergillus tubingensis. Its homology to Aspergillus tubingensis AY820009 was 99%. The mean UON-biotransformation rate was 94. 4% after two weeks cultivation at pH 6. 0 and 30℃. The relative molecular weight of biotransformation product analyzed by HPLC-MS was 314.4, which was 18. 1 larger than that of DON (296.3). NJA - 1 could tolerate the maximum 40 mg· L~(-1) DON in inorganic salt media. The result showed that NJA - 1 could to be used for DON biotransformation.关键词
脱氧雪腐镰刀菌烯醇/生物转化/分离/鉴定/β-tubulin基因/塔宾曲霉Key words
deoxynivalenol/biotransformation/isolation/identification/β-tuhulin gene/Aspergillus tubingensis分类
农业科技引用本文复制引用
何成华,樊彦红,刘国芳,张海彬..一株脱氧雪腐镰刀菌烯醇转化菌的分离、鉴定及其转化效果研究[J].南京农业大学学报,2009,32(4):143-147,5.基金项目
国家自然科学基金项目(30471277) （30471277）
教育部博士点基金项目(20040307043) （20040307043）
江苏省科技攻关项目(BE2004317) （BE2004317）
