南京医科大学学报（自然科学版）2009，Vol.29Issue(11)：1525-1529,1594,6.
非小细胞肺癌组织中HIF-1α和BNIP3的表达及相关性研究
Expression of hypoxia inducible factor-1α and BNIP3 in human non-small cell lung cancer and their correlation
王群 1刘季春1
作者信息
- 1. 南昌大学第一附属医院心胸外科,江西,南昌,330006
- 折叠
摘要
Abstract
Objective:To investigate the expression of hypoxia inducible factor-la (HIF-1α) and the Bcl-2/adenovirus E1B 19 kDa-interacting protein 3 (BNIP3) in human non-small cell lung carcinoma(NSCLC) and their correlation. Methods:The expression of BNIP3 and HIF-1α in 45 tumor samples,45 para-cancer tissues and 14 control normal lung tissues were examined by reverse tran-scription-polymerase chain reaction analyses (RT-PCR) and SP immunohistochemical method. Multivariable analysis was used to analyze the correlation of the HIF-la and BNIP3 expression on histological types,differentiation,TNM clinical staging,lymphatic metastasis, age and cigarette smoking. Results:Compared with the control group,the mRNA levels of HIF-1α and BNIP3 in lung carcinoma tissues were much higher. There was a significant difference between tumors and the control (P < 0.01). BNIP3 mRNA levels were positively correlated with HIF-1α levels (r=0.68,P < 0.01). In lung carcinoma,the positive expression rates of HIF-1α and BNIP3 proteins were 53.3% and 66.7% respectively,which were higher than those in para-cancer tissues and normal lung tissues (P < 0.01). The positive correlation between HIF-1α and BNIP3 expression was highly significant(r,=0.38,P < 0.01). The expression of HIF-1α was correlated with histological types and TNM clinical staging (P < 0.05). No significant correlation was found between BNIP3 expression and patients' clinicopathologic parameters. Conclusion:The high expression levels of HIF-1α and BNIP3 were closely related in non-small cell lung carcinomas,both of them played important roles in the carcinogenesis and development of lung carcinoma.关键词
非小细胞肺癌/HIF-1α/BNIP3Key words
non-small-cell lung cancer/ HIF-1α/ BNIP3分类
医药卫生引用本文复制引用
王群,刘季春..非小细胞肺癌组织中HIF-1α和BNIP3的表达及相关性研究[J].南京医科大学学报（自然科学版）,2009,29(11):1525-1529,1594,6.
