首页|期刊导航|南京医科大学学报（英文版）|Activation of angiotensin II type 1 receptors in the median preoptic nucleus induces a diuretic and natriuretic response in rats
南京医科大学学报（英文版）2009，Vol.23Issue(6)：410-414,5.
Activation of angiotensin II type 1 receptors in the median preoptic nucleus induces a diuretic and natriuretic response in rats
Activation of angiotensin II type 1 receptors in the median preoptic nucleus induces a diuretic and natriuretic response in rats
摘要
Abstract
Objective: To investigate the effect of activation of angiotensin II (AngII) type 1 (ATI) receptors in the median preoptic nucleus (MnPO) of rats on renal sodium excretion. Methods: After anesthesia, the rats were injected into the MnPO via an implanted cannula. Urine samples were collected via a bladder cannula, and the urine sodium concentration was assayed with flame spectrophotometry. The serum level of endogenous digitalis-like factor (EDLF) and Na+,K+-ATPase activity in the renal cortex tissue were assayed respectively with a radioimmunoassay and with an ammonium molybdophosphate-based kit. Results: Both the urinary volume and the sodium excretion peaked 60 min after Angll was administered into the MnPO. The responses were accompanied by an increase in serum EDLF and a decrease in Na+,K+-ATPase activity in the renal cortex. The responses of diuresis and natriuresis, as well as an increase in serum EDLF and a decrease in Na+,K+-ATPase activity in the renal cortex induced by MnPO adminstration with AngII were inhibited by pior treatment with the AngII receptor blocking agent losartan into the MnPO. Conclusion: These results suggest that activation of ATI receptors in the MnPO of rat induces diuretic and natriuretic responses. The responses are associated with an increase release of EDLF and with the inhibition of Na+,K+-ATPase activity in renal cortex tissue.关键词
angiotensin ATI receptor/ median preoptic nucleus/ natriuresis/ endogenous digitalis-like factor/ Na+,K+-ATPase/ ratKey words
angiotensin ATI receptor/ median preoptic nucleus/ natriuresis/ endogenous digitalis-like factor/ Na+,K+-ATPase/ rat分类
医药卫生引用本文复制引用
Yuan Gao ,Lei Luo,Hong Liu..Activation of angiotensin II type 1 receptors in the median preoptic nucleus induces a diuretic and natriuretic response in rats[J].南京医科大学学报（英文版）,2009,23(6):410-414,5.基金项目
This work was supported by the Science Foundation of Zhuhai Municipality (PC20052031) （PC20052031）
