农业环境科学学报2009，Vol.28Issue(11)：2401-2406,6.
粘土矿物固定化微生物对土壤中阿特拉津的降解研究
Biodegradation of Atrazine in Soils by Clay Minerals Immobilized a Degradation Bacterium
摘要
Abstract
Biodegradation of atrazine in soils by clay minerals immobilized a degradation bacterium were studied. The bacterium which was isolated from activated sludge by enrichment using atrazine as sole source of carbon and nitrogen was preliminarily identified as Ochrobac-trum sp., according to its physiological-biochemical characteristics, photos of ESEM and the similarity analysis of its 16S rDNA gene sequence. The strains growed well on clay minerals, and reached logarithmic phase at 48 h. The degradation rate of atrazine was larger signifi-cally when adding degradation bacteria into soils. The biodegradation capacity by immobilized microorganism on clay minerals was improved compared with free microorganism. The smaller the particle size of clay minerals, the more the biodegradation of atrazine. The effect of immobilized nano-clay minerals was better than corresponding clays. The degradation of atrazine were 42.47%, 46.19%, 56.31 % respectively, after adding free microorganism into vertisol, alfisol and red soil on 28 d. When adding immobilized microorganism by montmorillonite, nano-mont-morillonite, the degradation of atrazine were 52.16%, 63.97% in vertisol, 59.28%, 63.91% in alfisol, and 68.03%, 76.59% in red soil. The degradation of atrazine in the soil without microorganism was 29.16% in vertisol, 30.63% in alfisol, 41.47% in red soil. Atrazine degradation in soils could be described by first-order kinetic equation. The effect of degradation varied from different soils. The(t_(1/2) of atrazine was 36.9 d in red soil, 49.1 d in vertisol, and 55.0 d in alfisol without microorganism. When adding immobilized microorganism by nano-montmorillonite, half-life was 16.3 d, 25.3 d, 21.7 d respectively.关键词
阿特拉津/微生物同定化/粘土矿物/降解Key words
atrazine/ immobilization microorganism/ clay minerals/ degradation分类
资源环境引用本文复制引用
汪玉,王磊,司友斌,孟雪梅..粘土矿物固定化微生物对土壤中阿特拉津的降解研究[J].农业环境科学学报,2009,28(11):2401-2406,6.基金项目
国家863计划(2006AA06Z356) （2006AA06Z356）
国家973计划(2007CB936604):安徽省科技攻关计划(07010302212) （2007CB936604）
安徽省人才开发资金(2007Z014) （2007Z014）
