热带作物学报2009，Vol.30Issue(10)：1488-1494,7.
龙眼胚性愈伤组织限制生长保存及其染色体数目变异
Minimal Growth Conservation and Variation of Chromosome Number of Longan Embryogenic Callus
摘要
Abstract
'Honghezi' Longan(Dimocarpus longan 'Honghezi') was used to study the effects of mannitol and inositol cross-culture, sucrose, agar, salt, PP333 and light quality on the minimal growth of longan embryogenic callus(EC) for conservation. The results showed that the combined addition of 20 g/L mannitol and 0.1 g/L inositol with the inoculation amount of 0.1 g per bottle, prolonged the subculture cycle to more than 70 days. At the same time, the long cultivars 'Honghezi', 'Songfengben' and 'Julong 105' were used to investigate the variation of chromosome number of longan embryogenic callus during somatic embryogenesis. The results indicated that the chromosome variation occurred at the embryogenic cell stage and then tended to be stable.关键词
龙眼/胚性愈伤组织/限制生长保存/染色体变异Key words
longan/ embryogenic callus/ minimal growth conservation/ chromosome variation.分类
农业科技引用本文复制引用
林秀莲,赖钟雄..龙眼胚性愈伤组织限制生长保存及其染色体数目变异[J].热带作物学报,2009,30(10):1488-1494,7.基金项目
国家科技支撑计划项目(No.2007BAD07801),福建省科技平台建设项目(No 2008N2001)资助. （No.2007BAD07801）
