热带作物学报2009，Vol.30Issue(9)：1301-1307,7.
广西主要杧果资源遗传关系的SSR分析
Analysis of Genetic Diversity of Major Accessions of Mango (Mangifera indica L.) in Guangxi Using SSR Markers
摘要
Abstract
A total of 14 SSRs were used to amplify 63 alleles from 48 major mango accessions available in Guangxi to analyze their genetic diversity and phylogeny. All the primers produced clear and strong expected amplifications. The number of putative alleles was 3 to 6 per locus with an average number of alleles (A) being 4.5 and an effective number of alleles (Ne) being 2.66. The polymorphism information content (PIC) per locus ranged between 0.192 9 and 0.686 3, averaging 0.533 7. The observed (Ho) and expected(He) heterozygosities averaged 0.424 1 and 0.594 3, respectively. Genetic similarity coefficients of all the accessions ranged from 0.455 9 to 0.941 2, averaging 0.705 3, with the minimum between mango 'Guire 06 -3' and ' Guire 06 -5', and the maximum between ' Keitt' and ' Red Keitt' and between ' Yuwenmang' and ' Mitaomang'. UPGMA cluster analysis grouped the mango accessions to 5 groups according to their origin and their classification reflecting the pedigree of the cultivars. The clustering results showed that interspecific genetic relation of all the mango accessions was not related with the nucellar embryony (either monoembryonic or polyembryonic) and that most of the mango accessions in Guangxi were progenies of crosses and seedlings of cultivars Irwin, Okrong, Carabo and Neelum.关键词
杧果/SSR/遗传关系/聚类分析Key words
mangos/simple sequence repeats/genetic relationship/cluster analysis.分类
农业科技引用本文复制引用
王静毅,王家保,武耀廷,黄国弟,周俊岸,李日旺..广西主要杧果资源遗传关系的SSR分析[J].热带作物学报,2009,30(9):1301-1307,7.基金项目
国家科技基础条件平台重点项目(No.2005DKA21000-5)资助. （No.2005DKA21000-5）
