水产学报2009，Vol.33Issue(6)：980-986,7.
草鱼LPL基因的表达及饥饿和再投喂对其影响
Study on the LPL gene expression and the influcence of fasting and refeeding on it in grass carp, Ctenopharyngodon idellas
摘要
Abstract
For the better understanding about the mechanism of lipid metabolism at the molecular level in grass carp Ctenopharyngodon idellas, the partial cDNA sequence of lipoprotein lipase (LPL) gene was cloned (GenBank accession number FJ612596), and its expression in different organs, adipocytes, as well as the influence of fasting and refeeding on it in hepatopancrease were studied by means of semi-quantitative (SQ-RT-PCR . The results showed that the obtained cDNA sequence was 360 bp in length, and the homology is 70% - 89% with other species. LPL gene is widely expressed in hepatopancreas, muscle, heart, gill, intrapaneal fat body (IPF),mature adipocytes and stromal vascular fraction (SVF) cells , with the highest expression level in IPF, lowest one in gill among the organs (P <0. 05), and higher level in mature adipocytes, compared to SVF cells (P <0. 05). On the other hand, LPL gene expression level significantly increased in hepatopancreas after 48 h fasting and retrieved to the same level as 0 h after 12 h refeeding (P <0.05). It could be concluded that LPL gene is wildely expressed in various organs and at the initiation and final differentiation stage of adipocyte, regulated by the energy expenditure status, which implies its important role in lipid metabolism in grass carp. The further study on the modification mechanism of LPL gene is required in fish.关键词
草鱼/脂蛋白脂酶/基因表达/饥饿Key words
grass carp (Ctenopharyngodon idellas)/ lipoprotein lipase/ gene expression/ fasting分类
生物科学引用本文复制引用
吉红,苏尚顺,刘茜,曹艳姿,杨公社,林亚秋,奥宏海..草鱼LPL基因的表达及饥饿和再投喂对其影响[J].水产学报,2009,33(6):980-986,7.基金项目
国家自然科学基金项目(30771667) （30771667）
西北农林科技大学青年学术骨干支持计划项目 （）
西北农林科技大学博士后流动站项目 （）
