摘要
Abstract
The polymorphism distributions of 15 STR loci (D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, vWA, TPOX, D18S51, D5S818 and FGA) were investigated in a Hunan Han population using AmpFlSTR indentifiler kit, GeneAmp PCR system 9700 and ABI 310 genetic analyzer. Gene frequency, heterozygosity (H), polymorphism information content (PIC), power of discrimination (DP) and probability of paternity exclusion (PE) were calculated, and all loci were tested for Hardy-Weinberg equilibrium. Results indicate that the gene frequency of these 15 STR loci is in Hardy-Weinberg equilibrium. The H is at 0.593～0.900, PIC is at 0.54～0.85, DP is at 0.780～ 0.963, PE is at 0.282～0.785. Cumulative DP of the 15 STR is (1～1.6×10~(-17)) ＞ 0.999 999 99, and the cumulative PE is 0.999 999 5. Therefore, the 15 STR loci used in this study are highly polymorphic in Hunan Han population and can be applied to population study, individual identification and paternity testing in forensic science.关键词
短串联重复序列/遗传多态性/法医学Key words
short tandem repeat/ genetic polymorphism/ forensic science分类
生物科学
