生命科学研究2009，Vol.13Issue(5)：418-421,4.
香蕉MuMADS1基因表达产物的亚细胞定位
Subcellular Localization of Expression Products of Banana MuMADS1 Gene
摘要
Abstract
To investigate the regulation of MuMADS1 genes in banana (Musa acuminate L. AAA group cv. Brazilian.) fruit development and postharvest ripening, a MADS-box gene from banana fruit was isolated. Bioinformatics analysis show that, MuMADS1 gene encoding the protein as a transcription factor may be located in the nucleus. Our previous work demonstrated that it is up-regulated in the early stage of fruit ripening and plays roles in the upstream of ethylene signaling, which is associated with flower developing, fruit developing and ripening. In order to further study its function, a plant expression vector pCAMBIA1304-MuMADS1 with a green fluorescent protein was constructed and transformed into onion epidermal cells by particle bombardment method. The result of transient expression showed that MuMADS1 gene was located in the nucleus which was in line with the characteristics of transcription factor.关键词
香蕉/MuMADS1表达栽体/基因枪转化/亚细胞定位Key words
Banana(Musa.spp.)/ MuMADS1 plant expression vector/ particle bombardment transformation/ subcelluar localization分类
生物科学引用本文复制引用
周雪莉,王园,刘菊华,金志强,迟光红,徐碧玉..香蕉MuMADS1基因表达产物的亚细胞定位[J].生命科学研究,2009,13(5):418-421,4.基金项目
国家自然科学基金资助项目(30760153) （30760153）
中央级公益性科研院所基本科研业务费专项资金资助项目(ITBBZD0733) （ITBBZD0733）
